Imbred corn line LH301

ABSTRACT

An inbred corn line, designated LH301, is disclosed. The invention relates to the seeds of inbred corn line LH301, to the plants of inbred corn line LH301 and to methods for producing a corn plant produced by crossing the inbred line LH301 with itself or another corn line. The invention further relates to hybrid corn seeds and plants produced by crossing the inbred line LH301 with another corn line.

BACKGROUND OF THE INVENTION

The present invention relates to a new and distinctive corn inbred line,designated LH301. There are numerous steps in the development of anynovel, desirable plant germplasm. Plant breeding begins with theanalysis and definition of problems and weaknesses of the currentgermplasm, the establishment of program goals, and the definition ofspecific breeding objectives. The next step is selection of germplasmthat possess the traits to meet the program goals. The goal is tocombine in a single variety or hybrid an improved combination ofdesirable traits from the parental germplasm. These important traits mayinclude higher yield, resistance to diseases and insects, better stalksand roots, tolerance to drought and heat, and better agronomic quality.

Choice of breeding or selection methods depends on the mode of plantreproduction, the heritability of the trait(s) being improved, and thetype of cultivar used commercially (e.g., F₁ hybrid cultivar, purelinecultivar, etc.). For highly heritable traits, a choice of superiorindividual plants evaluated at a single location will be effective,whereas for traits with low heritability, selection should be based onmean values obtained from replicated evaluations of families of relatedplants. Popular selection methods commonly include pedigree selection,modified pedigree selection, mass selection, and recurrent selection.

The complexity of inheritance influences choice of the breeding method.Backcross breeding is used to transfer one or a few favorable genes fora highly heritable trait into a desirable cultivar. This approach hasbeen used extensively for breeding disease-resistant cultivars. Variousrecurrent selection techniques are used to improve quantitativelyinherited traits controlled by numerous genes. The use of recurrentselection in self-pollinating crops depends on the ease of pollination,the frequency of successful hybrids from each pollination, and thenumber of hybrid offspring from each successful cross.

Each breeding program should include a periodic, objective evaluation ofthe efficiency of the breeding procedure. Evaluation criteria varydepending on the goal and objectives, but should include gain fromselection per year based on comparisons to an appropriate standard,overall value of the advanced breeding lines, and number of successfulcultivars produced per unit of input (e.g., per year, per dollarexpended, etc.).

Promising advanced breeding lines are thoroughly tested and compared toappropriate standards in environments representative of the commercialtarget area(s) for three years at least. The best lines are candidatesfor new commercial cultivars; those still deficient in a few traits areused as parents to produce new populations for further selection.

These processes, which lead to the final step of marketing anddistribution, usually take from eight to 12 years from the time thefirst cross is made. Therefore, development of new cultivars is atime-consuming process that requires precise forward planning, efficientuse of resources, and a minimum of changes in direction.

A most difficult task is the identification of individuals that aregenetically superior, because for most traits the true genotypic valueis masked by other confounding plant traits or environmental factors.One method of identifying a superior plant is to observe its performancerelative to other experimental plants and to a widely grown standardcultivar. If a single observation is inconclusive, replicatedobservations provide a better estimate of its genetic worth.

The goal of plant breeding is to develop new, unique and superior corninbred lines and hybrids. The breeder initially selects and crosses twoor more parental lines, followed by repeated selfing and selection,producing many new genetic combinations. The breeder can theoreticallygenerate billions of different genetic combinations via crossing,selfing and mutations. The breeder has no direct control at the cellularlevel. Therefore, two breeders will never develop the same line, or evenvery similar lines, having the same corn traits.

Each year, the plant breeder selects the germplasm to advance to thenext generation. This germplasm is grown under unique and differentgeographical, climatic and soil conditions, and further selections arethen made, during and at the end of the growing season. The inbred lineswhich are developed are unpredictable. This unpredictability is becausethe breeder's selection occurs in unique environments, with no controlat the DNA level (using conventional breeding procedures), and withmillions of different possible genetic combinations being generated. Abreeder of ordinary skill in the art cannot predict the final resultinglines he develops, except possibly in a very gross and general fashion.The same breeder cannot produce the same line twice by using the exactsame original parents and the same selection techniques. Thisunpredictability results in the expenditure of large research monies todevelop a superior new corn inbred line.

The development of commercial corn hybrids requires the development ofhomozygous inbred lines, the crossing of these lines, and the evaluationof the crosses. Pedigree breeding and recurrent selection breedingmethods are used to develop inbred lines from breeding populations.Breeding programs combine desirable traits from two or more inbred linesor various broad-based sources into breeding pools from which inbredlines are developed by selfing and selection of desired phenotypes. Thenew inbreds are crossed with other inbred lines and the hybrids fromthese crosses are evaluated to determine which have commercialpotential.

Pedigree breeding is used commonly for the improvement ofself-pollinating crops or inbred lines of cross-pollinating crops. Twoparents which possess favorable, complementary traits are crossed toproduce an F₁. An F₂ population is produced by selfing one or several F₁'s or by intercrossing two F l's (sib mating). Selection of the bestindividuals is usually begun in the F₂ population; then, beginning inthe F₃, the best individuals in the best families are selected.Replicated testing of families, or hybrid combinations involvingindividuals of these families, often follows in the F₄ generation toimprove the effectiveness of selection for traits with low heritability.At an advanced stage of inbreeding (i.e., F₆ and F₇), the best lines ormixtures of phenotypically similar lines are tested for potentialrelease as new cultivars.

Mass and recurrent selections can be used to improve populations ofeither self- or cross-pollinating crops. A genetically variablepopulation of heterozygous individuals is either identified or createdby intercrossing several different parents. The best plants are selectedbased on individual superiority, outstanding progeny, or excellentcombining ability. The selected plants are intercrossed to produce a newpopulation in which further cycles of selection are continued.

Backcross breeding has been used to transfer genes for a simplyinherited, highly heritable trait into a desirable homozygous cultivaror inbred line which is the recurrent parent. The source of the trait tobe transferred is called the donor parent. The resulting plant isexpected to have the attributes of the recurrent parent (e.g., cultivar)and the desirable trait transferred from the donor parent. After theinitial cross, individuals possessing the phenotype of the donor parentare selected and repeatedly crossed (backcrossed) to the recurrentparent. The resulting plant is expected to have the attributes of therecurrent parent (e.g., cultivar) and the desirable trait transferredfrom the donor parent.

Descriptions of other breeding methods that are commonly used fordifferent traits and crops can be found in one of several referencebooks (e.g., Allard, 1960; Simmonds, 1979; Sneep et al., 1979; Fehr,1987).

Proper testing should detect any major faults and establish the level ofsuperiority or improvement over current cultivars. In addition toshowing superior performance, there must be a demand for a new cultivarthat is compatible with industry standards or which creates a newmarket. The introduction of a new cultivar will incur additional coststo the seed producer, the grower, processor and consumer; for specialadvertising and marketing, altered seed and commercial productionpractices, and new product utilization. The testing preceding release ofa new cultivar should take into consideration research and developmentcosts as well as technical superiority of the final cultivar. Forseed-propagated cultivars, it must be feasible to produce seed easilyand economically.

Once the inbreds that give the best hybrid performance have beenidentified, the hybrid seed can be reproduced indefinitely as long asthe homogeneity of the inbred parent is maintained. A single-crosshybrid is produced when two inbred lines are crossed to produce the F₁progeny. A double-cross hybrid is produced from four inbred linescrossed in pairs (A×B and C×D) and then the two F₁ hybrids are crossedagain (A×B)×(C×D). Much of the hybrid vigor exhibited by F₁ hybrids islost in the next generation (F₂). Consequently, seed from hybridvarieties is not used for planting stock.

Corn is an important and valuable field crop. Thus, a continuing goal ofplant breeders is to develop stable, high yielding corn hybrids that areagronomically sound. The reasons for this goal are obviously to maximizethe amount of grain produced on the land used and to supply food forboth animals and humans. To accomplish this goal, the corn breeder mustselect and develop corn plants that have the traits that result insuperior parental lines for producing hybrids.

SUMMARY OF THE INVENTION

According to the invention, there is provided a novel inbred corn line,designated LH301. This invention thus relates to the seeds of inbredcorn line LH301, to the plants of inbred corn line LH301 and to methodsfor producing a corn plant produced by crossing the inbred line LH301with itself or another corn line. This invention further relates tohybrid corn seeds and plants produced by crossing the inbred line LH301with another corn line.

DEFINITIONS

In the description and tables which follow, a number of terms are used.In order to provide a clear and consistent understanding of thespecification and claims, including the scope to be given such terms,the following definitions are provided:

Predicted RM. This trait for a hybrid, predicted relative maturity (RM),is based on the harvest moisture of the grain. The relative maturityrating is based on a known set of checks and utilizes conventionalmaturity systems such as the Minnesota Relative Maturity Rating System.

MN RM. This represents the Minnesota Relative Maturity Rating (MN RM)for the hybrid and is based on the harvest moisture of the grainrelative to a standard set of checks of previously determined MN RMrating. Regression analysis is used to compute this rating.

Yield (Bushels/Acre). The yield in bushels/acre is the actual yield ofthe grain at harvest adjusted to 15.5% moisture.

Moisture. The moisture is the actual percentage moisture of the grain atharvest.

GDU Silk. The GDU silk (=heat unit silk) is the number of growing degreeunits (GDU) or heat units required for an inbred line or hybrid to reachsilk emergence from the time of planting. Growing degree units arecalculated by the Barger Method, where the heat units for a 24-hourperiod are: ##EQU1##

The highest maximum used is 86° F. and the lowest minimum used is 50° F.For each hybrid, it takes a certain number of GDUs to reach variousstages of plant development. GDUs are a way of measuring plant maturity.

Stalk Lodging. This is the percentage of plants that stalk lodge, i.e.,stalk breakage, as measured by either natural lodging or pushing thestalks determining the percentage of plants that break off below theear. This is a relative rating of a hybrid to other hybrids forstandability.

Root Lodging. The root lodging is the percentage of plants that rootlodge; i.e., those that lean from the vertical axis at an approximate30° angle or greater would be counted as root lodged.

Plant Height. This is a measure of the height of the hybrid from theground to the tip of the tassel, and is measured in centimeters.

Ear Height. The ear height is a measure from the ground to the ear nodeattachment, and is measured in centimeters.

Dropped Ears. This is a measure of the number of dropped ears per plot,and represents the percentage of plants that dropped an ear prior toharvest.

DETAILED DESCRIPTION OF THE INVENTION

Inbred corn line LH301 is a yellow dent corn with superiorcharacteristics, and provides an excellent parental line in crosses forproducing first generation (F₁) hybrid corn.

LH301 is a corn inbred line developed from the single cross ofCM105×CB59G was made and then backcrossed with CM105. This combination,CM105×CB59G)(CM105 was then selfed and the pedigree system of plantbreeding was used in the development of LH301. Yield, stalk quality,root quality, disease tolerance, late plant greenness, late plantintactness, ear retention, pollen shedding ability, silking ability andcorn borer tolerance were the criteria used to determine the rows fromwhich ears were selected.

Inbred corn line LH301 has the following morphologic and othercharacteristics (based primarily on data collected at Williamsburg,Iowa).

VARIETY DESCRIPTION INFORMATION

1. TYPE: Dent

2. REGION WHERE DEVELOPED: Northcentral U.S.

3. MATURITY:

    ______________________________________                                                             Days Heat Units                                          ______________________________________                                        From emergence to 50% of plants in silk:                                                             75     1320                                              From emergence to 50% of plants in pollen 74 1290                           ______________________________________                                         ##STR1##                                                                     1   4. PLANT:                                                             

Plant Height (to tassel tip): 207.8 cm (SD=18.70)

Ear Height (to base of top ear): 96.4 cm (6.86)

Average Length of Top Ear Internode: 14.5 cm (1.20)

Average number of Tillers: 0 (0)

Average Number of Ears per Stalk: 1.0 (0.00)

Anthocyanin of Brace Roots: Dark

5. LEAF:

Width of Ear Node Leaf: 8.7 cm (0.54)

Length of Ear Node Leaf: 85.9 cm (3.75)

Number of leaves above top ear: 6 (0.48)

Leaf Angle from 2nd Leaf above ear at anthesis to Stalk above leaf: 31°(8.90)

Leaf Color: Medium Green--Munsell Code 5 GY 4/4

Leaf Sheath Pubescence (Rate on scale from 1=none to 9=like peach fuzz):7

Marginal Waves (Rate on scale from 1=none to 9=many): 5

Longitudinal Creases (Rate on scale from 1=none to 9=many): 3

6. TASSEL:

Number of Lateral Branches: 7 (1.46)

Branch Angle from Central Spike: 46° (16.71)

Tassel Length (from top leaf collar to tassel top): 41.8 cm (4.74)

Pollen Shed (Rate on scale from 0=male sterile to 9=heavy shed): 6

Anther Color: Dark yellow--Munsell Code 2.5GY 7/4

Glume Color: Medium Green--Munsell Code 5GY 5/6

Bar Glumes: Absent

7a. EAR: (Unhusked Data)

Silk Color (3 days after emergency): Salmon--Munsell Code 5Y 7/6

Fresh Husk Color (25 days after 50% silking): Light Green--Munsell Code2.5GY 8/6

Dry Husk Color (65 days after 50% silking): Buff--Munsell Code 7.5YR 7/4

Position of Ear: Pendent

Husk Tightness (Rate on scale from 1=very loose to 9=very tight): 5

Husk Extension: Medium (<8 cm)

7b. EAR: (Husked Ear Data)

Ear Length: 16.6 cm (1.09)

Ear Diameter at mid-point: 37.0 mm (1.60)

Ear Weight: 74.9 gm (18.91)

Number of Kernel Rows: 14 (1.11)

Kernel Rows: Distinct

Row Alignment: Straight

Shank Length: 25.0 cm (5.80)

8. KERNEL: (Dried)

Kernel Length: 9.6 mm (0.54)

Kernel Width: 8.9 mm (0.45)

Kernel Thickness: 5.7 mm (0.62)

Round Kernels (Shape Grade): 87.8% (4.04)

Aleurone Color Pattern: Homozygous

Aleurone Color: White--Munsell Code 2.5Y 8/2

Hard Endosperm Color: Yellow--Munsell Code 2.5Y 7/10

Endosperm Type: Normal Starch

Weight per 100 kernels: 31.9 gm (0.38)

9. COB:

Cob Diameter at Mid-Point: 30.8 mm (2.0)

Cob Color: Red--Munsell code 5R 3/4

10. DISEASE RESISTANCE:

Rating [1=(most susceptible) through 9=(most resistant)]

2 Eyespot (Kabatiella zeae)

4 Helminthosporium Leaf Spot (Bipolaris zeicola) Race 3

4 Northern Leaf Blight (Exserohilum turcicum) Race 2

5 Southern Leaf Blight (Bipolaris maydis)

11. AGRONOMIC TRAITS:

3 Stay Green (at 65 days after anthesis) (Rate on scale from 1=worst to9=excellent)

0% Dropped Ears (at 65 days after anthesis)

0% Pre-anthesis Brittle Snapping

0% Pre-anthesis Root Lodging

0% Post-anthesis Root Lodging (at 65 days after anthesis)

This invention is also directed to methods for producing a corn plant bycrossing a first parent corn plant with a second parent corn plant,wherein the first or second corn plant is the inbred corn plant from theline LH301. Further, both first and second parent corn plants may befrom the inbred line LH301. Therefore, any methods using the inbred cornline LH301 are part of this invention: selfing, backcrosses, hybridbreeding, and crosses to populations. Any plants produced using inbredcorn line LH301 as a parent are within the scope of this invention.Advantageously, the inbred corn line is used in crosses with other cornvarieties to produce first generation (F₁) corn hybrid seed and plantswith superior characteristics.

As used herein, the term "plant" includes plant cells, plantprotoplasts, plant cell of tissue culture from which corn plants can beregenerated, plant calli, plant clumps, and plant cells that are intactin plants or parts of plants, such as pollen, flowers, kernels, ears,cobs, leaves, husks, stalks, and the like.

Tissue culture of corn is described in European Patent Application,Publication No. 160,390, incorporated herein by reference. Corn tissueculture procedures are also described in Green and Rhodes, "PlantRegeneration in Tissue Culture of Maize", Maize for Biological Research(Plant Molecular Biology Association, Charlottesville, Va. 1982), at367-372. Thus, another aspect of this invention is to provide for cellswhich upon growth and differentiation produce the inbred line LH301.

CM105, V3×B14³ one of the progenitors of LH301, is a public linedeveloped by the Canadian Agricultural Research Station at Mordem,Manitoba, Canada. The inbred was released to the public in 1970. CB59G,[(B14A×R168)(B59² ], the other progenitor of LH301, is a proprietaryinbred line of Cornelius Hybrid Seed Co., Bellevue, Iowa. Holden'sFoundation Seeds, LLC and Cornelius Hybrid Seed Co. have a writtencontract whereby Holden's is the exclusive marketer of CB59G. Holden'soffered CB59G to their customers for the first time in 1979. Thisagreement between Holden's and Cornelius also allows Holden's permissionto use CB59G for breeding.

LH301 is similar to CM105, however, there are numerous differencesincluding the ear shank length. The ear shank length of LH301 is longerthan the ear shank length of CM105. This distinguishing characteristicmakes the ears of LH301 appear longer and more "showy" than the ears ofCM105.

LH301 is an early season field corn inbred line that flowersapproximately one day later than CM105. As an inbred line in seedproduction, LH301 is best used as a pollinator or as a seed parent insisterline combinations. Hybrids containing LH301 are similar inmaturity to LH145 combinations. LH301 combinations are showy, withexcellent staygreen. The stalk and root strength of LH301 hybrids arevery good and the test weight of LH301 hybrids is high.

Some of the criteria used to select ears in various generations include:yield, stalk quality, root quality, disease tolerance, late plantgreenness, late season plant intactness, ear retention, pollen sheddingability, silking ability, and corn borer tolerance. During thedevelopment of the line, crosses were made to inbred testers for thepurpose of estimating the line's general and specific combining ability,and evaluations were run by the Williamsburg, Iowa Research Station. Theinbred was evaluated further as a line and in numerous crosses by theWilliamsburg and other research stations across the Corn Belt. Theinbred has proven to have a very good combining ability in hybridcombinations.

The inbred has shown uniformity and stability for all traits. It hasbeen self-pollinated and ear-rowed a sufficient number of generations,with careful attention to uniformity of plant type to ensurehomozygosity and phenotypic stability. The line has been increased bothby hand and sibbed in isolated fields with continued observations foruniformity. No variant traits have been observed or are expected inLH301.

TABLES

In the tables that follow, the traits and characteristics of inbred cornline LH301 are given in hybrid combination. The data collected on inbredcorn line LH301 is presented for the key characteristics and traits. Thetables present yield test information about LH301. LH301 was tested inseveral hybrid combinations at numerous locations, with two or threereplications per location. Information about these hybrids, as comparedto several check hybrids, is presented.

The first pedigree listed in the comparison group is the hybridcontaining LH301. Information for the pedigree includes:

1. Mean yield of the hybrid across all locations.

2. A mean for the percentage moisture (% M) for the hybrid across alllocations.

3. A mean of the yield divided by the percentage moisture (Y/M) for thehybrid across all locations.

4. A mean of the percentage of plants with stalk lodging (% Stalk)across all locations.

5. A mean of the percentage of plants with root lodging (% Root) acrossall locations.

6. A mean of the percentage of plants with dropped ears (% Drop).

7. A mean of the plant height (Plant Hgt) in centimeters.

8. A mean of the ear height (Ear Hgt) in centimeters

9. The number of locations indicates the locations where these hybridswere tested together.

The series of hybrids listed under the hybrid containing LH301 areconsidered check hybrids. The check hybrids are compared to hybridscontaining the inbred LH301.

The (+) or (-) sign in front of each number in each of the columnsindicates how the mean values across plots of the hybrid containinginbred LH301 compare to the check crosses. A (+) or (-) sign in front ofthe number indicates that the mean of the hybrid containing inbred LH301was greater or lesser, respectively, than the mean of the check hybrid.For example, a +4 in yield signifies that the hybrid containing inbredLH301 produced 4 bushels more corn than the check hybrid. If the valueof the stalks has a (-) in front of the number 2, for example, then thehybrid containing the inbred LH301 had 2% less stalk lodging than thecheck hybrid.

                  TABLE 1                                                         ______________________________________                                        OVERALL COMPARISONS                                                             LH168 × LH301 HYBRID VERSUS CHECK HYBRIDS                                          Mean             %    %    %    Plant                                                                              Ear                           Pedigree Yield % M Y/M Stalk Root Drop Hgt Hgt                              ______________________________________                                        LH168 ×                                                                          140    19.12  7.32 4    0    0    110  48                              LH301                                                                         (at 7 Loc's)                                                                  As Compared                                                                   To:                                                                           LH168 × +11 0.50 0.41 -5 0 0 +2 +2                                      LH145                                                                         LH168 × -2 0.69 -0.41 -6 0 0 +1 +1                                      LH146                                                                       ______________________________________                                    

                  TABLE 2                                                         ______________________________________                                        OVERALL COMPARISONS                                                             LH176 × LH301 HYBRID VERSUS CHECK HYBRIDS                                          Mean             %    %    %    Plant                                                                              Ear                           Pedigree Yield % M Y/M Stalk Root Drop Hgt Hgt                              ______________________________________                                        LH176 ×                                                                          147    18.33  7.99 6    0    0    112  49                              LH301                                                                         (at 6 Loc's)                                                                  As Compared                                                                   To:                                                                           Pioneer Brand -15 -1.98 0.03 -2 0 0 +3 +1                                     LH146 × -2 -0.78 0.23 -14 -2 0 0 +4                                     LH145)(LH176                                                                  LH176 × -2 -0.15 -0.05 -6 -2 0 -2 +4                                    LH146                                                                       ______________________________________                                    

                  TABLE 3                                                         ______________________________________                                        OVERALL COMPARISONS                                                             LH290 × LH301 HYBRID VERSUS CHECK HYBRIDS                                          Mean             %    %    %    Plant                                                                              Ear                           Pedigree Yield % M Y/M Stalk Root Drop Hgt Hgt                              ______________________________________                                        LH290 ×                                                                          139    19.25  7.20 4    1    0    116  50                              LH301                                                                         (at 4 Loc's)                                                                  As Compared                                                                   To:                                                                           LH146 × -7 -0.09 -0.35 -7 0 0 -1 +4                                     LH145)(LH290                                                                  LH300 × +11 0.08 0.54 -4 0 0 +9 +11                                     LH85                                                                          LH85 × +6 0.30 0.19 -15 0 0 +9 +10                                      CM105                                                                         LH176 × +1 1.24 -0.46 -12 +1 0 +1 +2                                    LH146                                                                         LH145 × +8 1.25 -0.06 -18 0 0 +5 +8                                     A641)(LH85                                                                    LH145 × -11 1.42 -1.12 -13 0 0 +5 +5                                    A641)(LH290                                                                   LH146 × -2 1.59 -0.73 -10 +1 0 +2 +4                                    LH145)(LH176                                                                  LH176 × -13 1.61 -1.40 +1 0 0 +16 +11                                   LH290                                                                         LH223 × -4 1.74 -0.90 -13 +1 0 +4 +7                                    LH225)(LH176                                                                ______________________________________                                    

                  TABLE 4                                                         ______________________________________                                        OVERALL COMPARISONS                                                             LH163 × LH301 HYBRID VERSUS CHECK HYBRIDS                                          Mean             %    %    %    Plant                                                                              Ear                           Pedigree Yield % M Y/M Stalk Root Drop Hgt Hgt                              ______________________________________                                        LH163 ×                                                                          121    18.33  6.61 2    1    0    105  41                              LH301                                                                         (at 8 Loc's)                                                                  As Compared                                                                   To:                                                                           LH146 × 0 -0.96 0.34 -5 0 0 +3 0                                        Lh145)(LH163                                                                  LH223 × +8 1.08 0.04 -3 +1 0 +7 +1                                      LH163                                                                       ______________________________________                                    

DEPOSIT INFORMATION

Inbred seeds of LH301 have been placed on deposit with the American TypeCulture Collection (ATCC), Manassas, Va., under Deposit Accession Number203295 on Sep. 30, 1998. A Plant Variety Protection Certificate is beingapplied for with the United States Department of Agriculture.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

What is claimed is:
 1. An inbred corn seed designated LH301 having ATCCaccession No.
 203295. 2. A plant or its parts produced by growing theseed of claim
 1. 3. Pollen of the plant of claim
 2. 4. An ovule of theplant of claim
 2. 5. A corn plant having the physiological andmorphological characteristics of the plant of claim
 2. 6. Tissue cultureof the seed of claim
 1. 7. Tissue culture of the plant of claim
 2. 8. Acorn plant regenerated from the tissue culture of claim 7, wherein saidcorn plant is capable of expressing all the physiological andmorphological characteristics of inbred corn line LH301.
 9. A method forproducing a hybrid corn seed comprising crossing a first inbred parentcorn plant with a second inbred parent corn plant and harvesting theresultant hybrid corn seed, wherein said first or second parent cornplant is the corn plant of claim
 2. 10. A hybrid seed produced by themethod of claim
 9. 11. A hybrid plant or its parts produced by growingsaid hybrid corn seed of claim
 10. 12. Seed produced from said hybridplant of claim
 11. 13. A method for producing a hybrid corn seedcomprising crossing an inbred plant according to claim 2 with another,different corn plant.
 14. A hybrid seed produced by the method of claim13.
 15. A hybrid plant or its parts produced by growing said hybrid cornseed of claim
 14. 16. Seed produced from said hybrid plant of claim 15.17. A corn plant regenerated from the tissue culture of claim 6, whereinsaid corn plant is capable of expressing all the physiological andmorphological characteristics of inbred corn line LH301.